- Effect of O-2 concentrations on Sulfolobus solfataricus P2 doi link

Auteur(s): Simon G., Walther Jasper, Zabeti Nathalie, Combet-Blanc Y., Auria R., van der Oost John, Casalot L.

(Article) Publié: Fems Microbiology Letters, vol. 299 p.255-260 (2009)

DOI: 10.1111/j.1574-6968.2009.01759.x

Sulfolobus solfataricus P2 was grown aerobically at various O-2 concentrations. Based on growth parameters in microcosms, four types of behavior could be distinguished. At 35% O-2 (v/v; gas phase), the cultures did not grow, indicating a lethal dose of oxygen. For 26-32% O-2, the growth was significantly affected compared with the reference (21%), suggesting a moderate toxicity by O-2. For 16-24% O-2, standard growth was observed. For 1.5-15% O-2, growth was comparable with the reference, but the yield on O-2 indicated a more efficient use of oxygen. These results indicate that S. solfataricus P2 grows optimally in the range of 1.5-24% O-2, most likely by adjusting its energy-transducing machinery. To gain some insight into control of the respiratory system, transcriptomes of the strain cultivated at different O-2 concentrations, corresponding to each behavior (1.5%, 21% and 26%), were compared using a DNA microarray approach. It showed differential expression of several genes encoding terminal oxidases, indicating an adaptation of the strain's respiratory system in response to fluctuating oxygen concentrations.